Luciferase anticorps

N° du produit ABIN5541890

Cet anticorps Lapin Polyclonal détecte spécifiquement Luciferase dans WB, IF, DB, EIA et ICC. Il présente une réactivité envers Photinus pyralis.

Aperçu rapide pour Luciferase anticorps (ABIN5541890)

Antigène: Luciferase

Reactivité: Photinus pyralis

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Luciferase est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunocytochemistry (ICC)

Détail du produit anti-Luciferase anticorps

Specificité: IgG fraction of Polyclonal Rabbit antiserum to Luciferase from Photinus pyralis. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Immunogène: Luciferase isolated and purified from Photinus pyralis. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: ELISA. Dot blot. Immunoblotting. Indirect Immunofluorescence. Working Dilutions for Non-precipitating antibody-binding techniques: 1/1000-1/10000. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice.

Restrictions: For Research Use only

Stockage

Buffer: Buffer System: PBS, pH 7.2 without preservatives and foreign proteins

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Luciferase

Antigène: Luciferase

Sujet: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

UniProt: P08659